Mühlhausen (official German long version Mühlhausen/Thüringen) is a city of 33,000 inhabitants in Thuringia, Germany. It is the capital of the Unstrut-Hainich district in the north-west of Thuringia, situated only 5 km (3 miles) north of Germany's accurate geographical centre (in the neighbouring municipality of Niederdorla) as well as 50 km (31 miles) north-west of Erfurt, 65 km (40 miles) east of Kassel and 50 km (31 miles) south-east of Göttingen.
Mühlhausen was first mentioned in 967 and became one of the most important cities in central Germany during the later Middle Ages. In the mid-13th century, it became a Freie Reichsstadt, an independent and republican self-ruled member of the Holy Roman Empire, controlling an area of approximately 220 square kilometres (85 sq mi) and 19 regional villages. Due to its long-distance trade, Mühlhausen was prosperous and influential with a population of 10,000 around 1500. Because it was spared from later destruction, Mühlhausen today has a great variety of historical buildings with one of the largest medieval city centres remaining in Germany, covering a surface of more than 50 hectares within the inner city wall and approximately 200 hectares overall. There are eleven Gothic churches, several patricians’ houses and a nearly completely preserved fortification.
Johann Sebastian Bach worked as the city's organist in 1707/08. The theologian Thomas Müntzer, a leading person in the German Peasants' War, gave sermons here and was executed in front of the city. John A. Roebling, the constructor of the Brooklyn Bridge and Friedrich August Stüler, an influential architect in mid-19th-century Prussia, were born in Mühlhausen.
After 1815, Mühlhausen was part of the newly created Prussian Province of Saxony, where the Mühlhausen district was established. In 1831, the Mühlhausen-born civil engineer John A. Roebling, later constructor of the Brooklyn Bridge, emigrated to the United States. The era of industrialisation and urbanisation reached Mühlhausen relatively late, around 1870, when the city was connected to the railway by a line from Gotha to Leinefelde. In 1892, Mühlhausen became an independent city district. In 1898, the municipal power station was founded and the tramway network started its operation in Mühlhausen. Major industries were textiles and engineering.
Between 1910 and 1917, the provincial hospital of Pfafferode was built on the western edge of the city, one of the largest hospitals in the Prussian Province of Saxony.

Mühlhausen is located within the Thuringian Basin, a flat and fertile area, on the Unstrut river on the eastern edge of the Hainich hills.